Researchers from Columbia and Harvard have successfully used AI tools to engineer a portion of the E. coli ribosome that functions without isoleucine, one of the 20 standard amino acids. By replacing isoleucine with similar amino acids such as valine through iterative testing and deep-learning software, the team created an isoleucine-free small subunit in the bacteria. Although these engineered cells survived, they experienced slower growth rates than unmodified strains. This research investigates the possibility of simplified genetic codes and offers clues about how early life may have operated with fewer amino acids.
